Trypan Blue is widely used in biological and medical research as a vital stain to distinguish between live and dead cells. Its primary application is in cell viability assays, where it selectively stains dead cells with a blue color, allowing researchers to assess cell health and survival rates. This is particularly useful in cell culture experiments, where monitoring cell viability is crucial for maintaining healthy cultures and optimizing experimental conditions. In addition to cell viability assays, Trypan Blue is employed in tissue engineering and regenerative medicine to evaluate the integrity of cell membranes. It is also used in ophthalmology for diagnostic purposes, such as staining the anterior capsule of the lens during cataract surgery to enhance visibility and precision. Furthermore, it plays a role in microbiology for detecting and counting viable cells in microbial populations. Its ability to selectively stain non-viable cells makes it an essential tool in various scientific and medical applications.