Used as a reference standard in analytical chemistry for the accurate identification and quantification of trans-1,4-Dichloro-2-butene in environmental and industrial samples. Commonly employed in gas chromatography (GC) and high-performance liquid chromatography (HPLC) to calibrate instruments and validate test methods. Ensures reliable detection of impurities or degradation products in chemical manufacturing processes. Also utilized in air and water quality monitoring to assess contamination levels and ensure regulatory compliance. Its standardized concentration supports consistent results in quality control and research laboratories.