Used in biomedical research to quantify Dickkopf-1 (Dkk-1) protein levels in human serum, plasma, and cell culture supernatants. It plays a key role in studying bone metabolism disorders such as osteoporosis, as Dkk-1 is a negative regulator of the Wnt signaling pathway, which controls bone formation. The kit is also applied in cancer research, particularly in multiple myeloma and other malignancies where Dkk-1 contributes to tumor progression and bone lesions. Additionally, it is used in autoimmune and inflammatory disease studies, including rheumatoid arthritis, to assess Dkk-1 involvement in joint destruction and disease activity. The assay enables high-throughput, accurate measurement of Dkk-1, supporting both clinical investigations and drug development targeting Wnt pathway modulation.