Used as a fluorescent probe in biochemical assays to detect metal ions, particularly zinc and copper, in cellular environments. Its high sensitivity and selectivity make it suitable for imaging intracellular ion distribution in live cells. Also applied in environmental monitoring to quantify trace metal contaminants in water samples. Exhibits strong emission upon binding, enabling real-time detection in flow cytometry and fluorescence microscopy. Stable under physiological conditions, allowing prolonged observation without significant signal degradation.