Used as a fluorescent probe for detecting zinc ions in biological systems. It shows high selectivity for Zn²⁺ over other metal ions, making it suitable for imaging labile zinc pools in live cells. The probe exhibits a turn-on fluorescence response upon binding to zinc, allowing for sensitive detection using fluorescence microscopy or spectroscopy. Commonly applied in neuroscience research to study zinc's role in synaptic transmission and neuronal signaling. Also utilized in studies related to insulin secretion, where zinc is co-released with insulin from pancreatic β-cells. Its cell permeability and low cytotoxicity support use in real-time monitoring of intracellular zinc dynamics.