Zymogram Renaturation Buffer (10x) is primarily used in the process of renaturing proteins after electrophoresis, particularly in zymography techniques. It is essential for restoring the native conformation and activity of enzymes, such as proteases, that have been denatured during the separation process on polyacrylamide gels. This buffer is applied during the incubation step, allowing enzymes to refold into their active forms, which is crucial for detecting enzymatic activity through subsequent staining or visualization methods. Its use is critical in studying enzyme function, identifying specific protease activities, and analyzing protein interactions in biological samples.