Acridine Yellow G is primarily used as a fluorescent dye in biological staining and microscopy. It is particularly effective in highlighting cellular structures, such as nucleic acids, due to its strong binding affinity to DNA and RNA. This dye is widely applied in fluorescence microscopy to visualize and study the organization and behavior of chromosomes during cell division. Additionally, it is utilized in histology for staining tissues to differentiate cellular components under a microscope. Acridine Yellow G also finds application in flow cytometry, where it helps in analyzing and sorting cells based on their fluorescence properties. Its ability to emit bright fluorescence under UV light makes it a valuable tool in various diagnostic and research applications.