Z-GLY-GLY-OSU is widely used in peptide synthesis as an active ester reagent. Its primary application is in the coupling of amino acids or peptides, facilitating the formation of peptide bonds efficiently. It is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase peptide synthesis, where it acts as a building block to introduce glycine residues into peptide chains. The compound is valued for its stability and reactivity, making it a reliable choice for researchers and chemists working on the development of peptides for pharmaceutical, biochemical, and biomedical studies. Additionally, it is employed in the preparation of peptide-based drugs, enzyme substrates, and other biologically active peptides.