Widely used in biochemistry and molecular biology for affinity purification. Binds tightly to biotin, allowing efficient capture and isolation of biotinylated molecules such as proteins, nucleic acids, or complexes from crude samples like cell lysates. Commonly applied in pull-down assays to study protein-protein interactions. Also used in immunoprecipitation, ELISA, and detection methods where biotin-labeled probes are employed. The agarose bead format enables easy handling and separation by centrifugation. The beads can be regenerated and reused after appropriate cleaning, providing cost-effectiveness in laboratory settings. Ideal for both small-scale research and larger purification workflows due to high specificity and low non-specific binding.