DMT-Cl is widely used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protecting group for the 5'-hydroxyl group during the solid-phase synthesis of DNA and RNA. This compound ensures selective deprotection, allowing for precise control over the assembly of nucleotide chains. Its application is critical in the production of primers, probes, and other oligonucleotides used in PCR, sequencing, and gene editing technologies like CRISPR. Additionally, DMT-Cl is employed in the preparation of modified nucleotides for therapeutic and diagnostic purposes, contributing to advancements in personalized medicine and biotechnology.