CI-966 hydrochloride is primarily used in research settings to study the mechanisms of neurotransmitter transport in the brain. It acts as a potent and selective inhibitor of the GABA uptake system, making it a valuable tool for understanding the role of GABA in neurological and psychiatric disorders. Researchers utilize it to explore conditions such as anxiety, epilepsy, and depression, where GABAergic signaling is often disrupted. Additionally, it helps in the development of potential therapeutic agents targeting GABA transporters. Its application extends to preclinical studies aimed at elucidating the pharmacological properties of GABA-related pathways and their impact on brain function.