DL-Propargylglycine is primarily used in biochemical research as an inhibitor of the enzyme cystathionine gamma-lyase (CSE). This enzyme is involved in the production of hydrogen sulfide (H₂S) in biological systems. By inhibiting CSE, DL-Propargylglycine helps researchers study the role of hydrogen sulfide in various physiological and pathological processes, such as inflammation, cardiovascular function, and neurotransmission. It is also employed in studies exploring the therapeutic potential of modulating H₂S levels in diseases like hypertension, diabetes, and neurodegenerative disorders. Additionally, DL-Propargylglycine is utilized in metabolic pathway analysis to understand the regulation of sulfur-containing amino acids and their derivatives.