3-O-methyl-N-acetyl-D-Glucosamine (3-O-methyl-GlcNAc) is primarily utilized in biochemical and biomedical research as a specialized tool for studying glycosylation processes and carbohydrate metabolism. It serves as a substrate or inhibitor in enzymatic assays to investigate the activity of glycosyltransferases and glycosidases, which are crucial in understanding cellular signaling and protein modification. In glycobiology, it aids in exploring the structural and functional roles of glycans in cell recognition, immune response, and pathogen interactions. Additionally, it is used in the development of glycan-based therapeutics and diagnostics, particularly in cancer research, where altered glycosylation patterns are a hallmark of disease progression. Its unique methylated structure also makes it valuable in synthetic chemistry for designing and synthesizing novel glycoconjugates.