Used as a stable isotope-labeled compound in metabolic studies to trace biochemical pathways, particularly in research involving acetyl-CoA metabolism and the citric acid cycle. Its isotopic labeling allows precise tracking of carbon atoms in cellular processes, making it valuable in NMR spectroscopy and mass spectrometry analyses. Commonly applied in pharmaceutical research, environmental science, and studies of fatty acid synthesis where high isotopic purity is required. Also employed in the development and validation of analytical methods for quantifying metabolic flux in biological systems.