Biotin-PEG2-OH is widely used in bioconjugation and biochemical research due to its ability to link biotin to various molecules or surfaces. The PEG (polyethylene glycol) spacer enhances solubility and reduces steric hindrance, making it ideal for attaching biotin to proteins, peptides, or other biomolecules. This compound is particularly valuable in creating biotinylated probes for applications such as ELISA, Western blotting, and affinity chromatography, where biotin-streptavidin interactions are utilized for detection or purification. Additionally, it is employed in the development of drug delivery systems, where biotinylation can target specific cells or tissues, improving the efficiency of therapeutic agents. Its versatility and compatibility with biological systems make it a key tool in molecular biology and biomedical research.