Biotin-PEG3-MAL is widely used in bioconjugation and biochemical research. It serves as a linker to attach biotin to proteins, peptides, or other molecules, enabling their detection or purification through streptavidin-biotin interactions. The PEG3 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. This compound is particularly valuable in immunoassays, fluorescence labeling, and targeted drug delivery systems. It also aids in studying protein-protein interactions and cellular processes by facilitating the attachment of biotinylated probes to biomolecules. Its maleimide group allows for specific conjugation with thiol-containing compounds, ensuring precise and stable modifications.