Primarily used as a buffer agent in biochemical and molecular biology applications, it helps maintain stable pH levels during enzymatic reactions and protein studies. It is particularly effective in electrophoresis and chromatography techniques, ensuring optimal conditions for separation and analysis of biomolecules. Additionally, it is utilized in diagnostic assays and research involving nucleic acids, where precise pH control is critical for accurate results. Its sulfonic acid group enhances solubility in aqueous solutions, making it suitable for various laboratory procedures.