HEPPSO is widely used as a buffering agent in biochemical and molecular biology research due to its effective pH stabilization properties in the range of 7.1 to 8.5. It is particularly useful in cell culture media, enzyme assays, and protein purification processes, where maintaining a stable pH is critical for optimal activity and stability of biological molecules. Its zwitterionic nature minimizes interference with biochemical reactions, making it suitable for sensitive applications. Additionally, HEPPSO is employed in electrophoresis and chromatography techniques to ensure consistent and reliable results. Its low toxicity and compatibility with biological systems further enhance its utility in various laboratory settings.