Herbimycin C is primarily utilized in research settings to study cellular processes and signaling pathways. It acts as a potent inhibitor of heat shock protein 90 (Hsp90), a chaperone protein involved in the stabilization and activation of numerous client proteins, including kinases and transcription factors. By disrupting Hsp90 function, it helps researchers investigate the role of this protein in cancer, neurodegenerative diseases, and other conditions. Additionally, it is used to explore mechanisms of protein folding, degradation, and stress responses in cells. Its application in experimental studies has provided insights into potential therapeutic targets for diseases associated with misfolded or dysregulated proteins.