Glyoxime is primarily used as a chelating agent in analytical chemistry to detect and quantify metal ions, particularly nickel and palladium. It forms stable complexes with these metals, enabling their separation and identification in various samples. In organic synthesis, glyoxime serves as a precursor for the preparation of heterocyclic compounds, such as oxadiazoles and triazoles, which are valuable in pharmaceutical and agrochemical industries. Additionally, it is employed in the development of corrosion inhibitors, where it helps protect metal surfaces from degradation in harsh environments. Its ability to act as a ligand in coordination chemistry also makes it useful in catalysis and material science applications.