HPLC Analysis of Niacin (Nicotinic Acid) Impurities in Niacinamide
- Product Code: 35536
Reverse-phase HPLC-UV method for quantification of nicotinic acid (niacin) as a process impurity in niacinamide (nicotinamide) powder.
description Sample Requirement
Solid (niacinamide powder) 0.1 grams
insert_drive_file Report of Previously Tested Sample
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timeline Service Steps
| Step | Procedure | Expected Result |
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1
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Prepare phosphate... |
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Clear buffer at pH 3.0.
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2
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Prepare mobile... |
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Homogeneous mobile phase.
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3
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Sample: Weigh... |
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Clear solution without particulates.
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4
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Standard: Dissolve... |
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Clear standard solution.
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5
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Inject 20... |
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Nicotinic acid Rt ~4.5 min, niacinamide ~7.5 min; impurity <=0.1% w/w relative to standard.
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