Cholesterol myristate is widely used in the formulation of cosmetic and skincare products. It acts as an emollient, helping to soften and smooth the skin by forming a protective barrier that locks in moisture. This makes it particularly effective in creams, lotions, and lip balms designed for dry or sensitive skin.

In the pharmaceutical industry, it serves as an excipient in drug delivery systems. Its lipid-like properties enable it to enhance the solubility and stability of active pharmaceutical ingredients, particularly in topical formulations. Additionally, it is used in research as a standard or reference material in lipid studies, aiding in the analysis of lipid metabolism and membrane dynamics.

In industrial applications, cholesterol myristate is utilized in the production of liquid crystals, which are essential components in display technologies such as LCD screens. Its ability to form stable mesophases makes it a valuable material in this field.