5-Methylumbelliferone is widely used as a fluorescent probe in biochemical and cellular studies. It is particularly valuable in enzyme assays, where it helps detect the activity of enzymes such as β-glucuronidase and arylsulfatase. Its fluorescence properties make it a useful tool for monitoring enzymatic reactions in real-time. Additionally, it is employed in research related to cancer and inflammation, as it has been studied for its potential to inhibit hyaluronan synthesis, which plays a role in tumor growth and metastasis. In dermatology, it is explored for its skin-lightening effects due to its ability to inhibit tyrosinase activity. Furthermore, it serves as a building block in the synthesis of various organic compounds and pharmaceuticals.