This chemical is widely used as a fluorescent dye in biological and biochemical research. It is particularly valuable for labeling cell membranes, measuring membrane potential, and studying membrane dynamics due to its ability to integrate into lipid bilayers. Its fluorescence properties, which are sensitive to changes in membrane potential, make it a useful tool in fluorescence microscopy and flow cytometry for visualizing and tracking cellular processes. Additionally, it is employed in the development of optical sensors and imaging techniques, aiding in the study of cellular interactions and signaling pathways. Its application extends to photophysical studies, where it helps in understanding energy transfer mechanisms and molecular interactions in various systems. It also finds use in clinical research for detecting abnormal or cancer cells through potential-sensitive imaging.