Used extensively in biological imaging and diagnostics due to its strong fluorescence in the near-infrared range. It is particularly valuable for labeling proteins, peptides, and nucleic acids, enabling detailed visualization of cellular processes. The compound is often employed in fluorescence microscopy, flow cytometry, and in vivo imaging studies. Its water solubility and reactive ester group make it ideal for conjugation with biomolecules without significantly altering their function. Additionally, it is utilized in drug delivery research to track the distribution and uptake of therapeutic agents in tissues.