DiOC16(3) perchlorate is widely used as a fluorescent dye in biological research, particularly for studying cell membranes and lipid dynamics. It is highly effective in labeling and visualizing live cells due to its ability to incorporate into lipid bilayers. This dye is commonly employed in fluorescence microscopy to track membrane potential and monitor changes in cellular morphology. Additionally, it is utilized in flow cytometry to analyze cell populations based on membrane properties. Its lipophilic nature makes it suitable for investigating membrane fluidity and organization in various cell types. Researchers also use it to study vesicle trafficking and membrane fusion processes in cellular systems.