6-Deoxy-D-Glucose is primarily used in biochemical research as a metabolic inhibitor. It is often employed to study cellular glucose uptake and metabolism, particularly in cancer research, where it helps understand how cancer cells utilize glucose compared to normal cells. This compound is also used in the investigation of glycolysis pathways, as its structure allows it to interfere with glucose metabolism, providing insights into metabolic regulation and enzyme functions. Additionally, it serves as a tool in microbiology to study bacterial sugar transport systems and their resistance mechanisms. Its application extends to the development of diagnostic techniques for metabolic disorders and the evaluation of potential therapeutic agents targeting glucose metabolism.