Used in peptide synthesis as a protected dipeptide building block, Ac-Ala-OMe serves as a model compound for studying peptide bond formation and enzyme activity. It is commonly employed in biochemical research to investigate protease kinetics and specificity due to the acetylated N-terminus and methyl ester C-terminus, which mimic natural peptide modifications. Its stability and solubility make it suitable for use in solution-phase synthesis and kinetic assays. Additionally, it acts as an intermediate in the preparation of larger peptide sequences and peptidomimetic drugs.