Ac-Gly-BoroPro is primarily utilized in the field of medicinal chemistry and biochemistry as a potent and selective inhibitor of proteases, particularly dipeptidyl peptidase IV (DPP-IV). Its boronic acid moiety enables it to form stable covalent interactions with the active site of the enzyme, making it a valuable tool for studying enzyme mechanisms and inhibition. This compound is often employed in research to investigate the role of DPP-IV in various physiological processes, including glucose metabolism and immune regulation. Additionally, it serves as a key structural template for the development of therapeutic agents targeting DPP-IV, which is relevant in the treatment of type 2 diabetes and other metabolic disorders. Its application extends to drug discovery programs where it aids in the optimization of inhibitor potency and selectivity.