AMPHOLINE is widely used in the field of biochemistry and molecular biology for isoelectric focusing (IEF), a technique used to separate proteins based on their isoelectric points. It serves as a carrier ampholyte, creating a stable pH gradient in polyacrylamide gels, enabling precise separation and analysis of proteins. This application is crucial in proteomics research, allowing scientists to study protein composition, identify biomarkers, and understand post-translational modifications. Additionally, AMPHOLINE is utilized in the purification of proteins and peptides, aiding in the preparation of highly pure samples for further analytical or functional studies. Its role in IEF makes it an essential tool in advancing research related to disease mechanisms, drug development, and cellular processes.