Used to measure the activity of phosphoenolpyruvate carboxykinase (PCK) in bovine biological samples such as serum, plasma, or tissue extracts. This assay is commonly applied in metabolic research, particularly in studies related to gluconeogenesis, energy metabolism, and liver function in cattle. It helps in diagnosing and understanding metabolic disorders like ketosis and fatty liver disease in dairy cows, especially during early lactation when metabolic demand is high. The kit enables high-throughput, accurate quantification, supporting both veterinary diagnostics and agricultural research aimed at improving livestock health and productivity.