Used in environmental toxicology studies to measure acetylcholinesterase inhibition in zebrafish, serving as a biomarker for neurotoxic effects of pesticides, heavy metals, and emerging contaminants. Enables high-throughput screening of chemical toxicity in aquatic ecosystems. Widely applied in pharmaceutical research and drug development to assess neuroactive compounds. Supports mechanistic studies in neuroscience due to zebrafish’s genetic similarity to humans. Provides a sensitive, quantitative method for monitoring enzyme activity in whole organisms, tissue homogenates, or cell extracts.