UV-VIS Trypsin Activity Measurement

  • Product Code: 35453

UV-VIS Trypsin Activity Measurement

฿2,990.00
Service Steps
Step Procedure Expected Result
1

50mM...

50mM phosphate buffer solution.

2

 0.02g...

2% trypsin standard solution.

3

Dilute...

trypsin standards solution with various concentration.

4

0.004349...

10 mmol of BAPA solutions.

5

Prepare...

1mmol (Working Reagent, WR) of BAPA solution.

6

30ml...

30% acetic acid solution.

7

120...

Reaction of the trypsin activity and Homogenous of the mixture.

8

The...

 Stopping the reaction for the trypsin activity and substrate

9

The...

Determination of the trypsin content 

10

Prepare...

Obtaining the standard curve with difference concentration.

11

Use...

the value of trypsin absorbance at the specific wavelength                            

the value of trypsin content in the sample .

You will receive a report for the the value of trypsin concentration (mg/ml or %) , the value of trypsin activity (U/g) fields when we provide this service

UV-VIS Trypsin Activity Measurement

UV-VIS Trypsin Activity Measurement

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Service Steps
Step Procedure Expected Result
1

50mM...

50mM phosphate buffer solution.

2

 0.02g...

2% trypsin standard solution.

3

Dilute...

trypsin standards solution with various concentration.

4

0.004349...

10 mmol of BAPA solutions.

5

Prepare...

1mmol (Working Reagent, WR) of BAPA solution.

6

30ml...

30% acetic acid solution.

7

120...

Reaction of the trypsin activity and Homogenous of the mixture.

8

The...

 Stopping the reaction for the trypsin activity and substrate

9

The...

Determination of the trypsin content 

10

Prepare...

Obtaining the standard curve with difference concentration.

11

Use...

the value of trypsin absorbance at the specific wavelength                            

the value of trypsin content in the sample .

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