UV-VIS Trypsin Activity Measurement

  • Product Code: 35453

UV-VIS Trypsin Activity Measurement

$139.78

science ชื่อตัวอย่าง

assignment คำแนะนำ

info โปรดระบุข้อกำหนดหรือเงื่อนไขเฉพาะสำหรับการทดสอบ

timeline ขั้นตอนการให้บริการ

ขั้นตอน ขั้นตอน ผลลัพธ์ที่คาดหวัง
1

50mM...

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50mM phosphate buffer solution.

2

 0.02g...

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2% trypsin standard solution.

3

Dilute...

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trypsin standards solution with various concentration.

4

0.004349...

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10 mmol of BAPA solutions.

5

Prepare...

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1mmol (Working Reagent, WR) of BAPA solution.

6

30ml...

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30% acetic acid solution.

7

120...

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Reaction of the trypsin activity and Homogenous of the mixture.

8

The...

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 Stopping the reaction for the trypsin activity and substrate

9

The...

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Determination of the trypsin content 

10

Prepare...

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Obtaining the standard curve with difference concentration.

11

Use...

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the value of trypsin absorbance at the specific wavelength                            

the value of trypsin content in the sample .

คุณจะได้รับรายงานสำหรับ the value of trypsin concentration (mg/ml or %) , the value of trypsin activity (U/g) ฟิลด์เมื่อเราให้บริการนี้

UV-VIS Trypsin Activity Measurement

UV-VIS Trypsin Activity Measurement

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Service Steps
Step Procedure Expected Result
1

50mM...

50mM phosphate buffer solution.

2

 0.02g...

2% trypsin standard solution.

3

Dilute...

trypsin standards solution with various concentration.

4

0.004349...

10 mmol of BAPA solutions.

5

Prepare...

1mmol (Working Reagent, WR) of BAPA solution.

6

30ml...

30% acetic acid solution.

7

120...

Reaction of the trypsin activity and Homogenous of the mixture.

8

The...

 Stopping the reaction for the trypsin activity and substrate

9

The...

Determination of the trypsin content 

10

Prepare...

Obtaining the standard curve with difference concentration.

11

Use...

the value of trypsin absorbance at the specific wavelength                            

the value of trypsin content in the sample .

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