Gln-AMS is primarily used in biochemical research as a potent inhibitor of glutamine-dependent enzymes. It is particularly valuable in studying the role of glutamine in cellular processes, such as metabolism and protein synthesis. Researchers utilize Gln-AMS to investigate the mechanisms of glutamine-addicted cancer cells, as it effectively blocks glutaminase, an enzyme critical for their survival. This makes it a useful tool in developing targeted cancer therapies. Additionally, Gln-AMS aids in exploring metabolic pathways and identifying potential drug targets in diseases where glutamine metabolism is dysregulated. Its application extends to understanding bacterial and fungal metabolism, providing insights into antimicrobial strategies.