PKM2-IN-3 is primarily utilized in research focused on cancer metabolism. It acts as a selective inhibitor of pyruvate kinase M2 (PKM2), an enzyme that plays a critical role in the metabolic reprogramming of cancer cells. By inhibiting PKM2, this compound disrupts the altered energy production pathways in cancer cells, potentially slowing their growth and proliferation. Researchers use PKM2-IN-3 to study the metabolic dependencies of tumors and to explore its potential as a therapeutic agent in cancer treatment. Its application is particularly relevant in understanding how targeting metabolic enzymes can lead to novel anti-cancer strategies.