Used in molecular biology for dephosphorylation of DNA and RNA ends to prevent self-ligation and facilitate ligation of new DNA fragments during cloning procedures, particularly in restriction enzyme cloning and vector preparation.

Employed as a reporter enzyme in biotechnology for detection in immunoassays such as ELISA, where it reacts with substrates to produce color or light signals for high-sensitivity measurement of antibodies or target proteins.

In diagnostics, serves as a marker enzyme in clinical assays to evaluate liver and bone disorders, such as liver disease, rickets, or bile duct obstruction, by detecting elevated levels in blood.

Widely utilized in research for protein detection in Western blotting and other blotting techniques.