DNase I is a recombinant enzyme derived from bovine pancreas (≥5000 units/mg protein). It is widely used in molecular biology to remove contaminating DNA from RNA and protein samples, helping prevent DNA interference during RNA purification and ensuring high-quality RNA for RT-PCR and other downstream applications. It is commonly used in cell culture processes to reduce viscosity caused by released DNA during cell lysis. Additionally, it is applied in chromatin immunoprecipitation (ChIP) assays to shear DNA into smaller fragments for analysis. Its high specificity for DNA makes it ideal for cleaning up enzymatic reactions without affecting RNA or proteins. It is frequently used in the preparation of DNA-free antibodies and vaccines. It is also effective in reducing biofilm formation in industrial and medical research settings due to its ability to degrade extracellular DNA in microbial communities.