Widely used in molecular biology for precise DNA cleavage at specific recognition sites, enabling efficient cloning and genetic engineering. Commonly applied in restriction digestion protocols to generate sticky ends for ligation into compatible vectors. Valued for high specificity and fast reaction times, supporting high-throughput workflows in gene assembly and DNA analysis. Also utilized in diagnostic assays and genome editing to verify constructs or excise fragments with accuracy. Stable under standard reaction conditions, making it suitable for use in multi-enzyme digests.