Used in biochemical assays to catalyze the phosphorylation of glucose and other hexoses, enabling glucose quantification in clinical diagnostics and research. Commonly applied in glucose assay kits, such as those for blood glucose monitoring, where it ensures high specificity and sensitivity. Also employed in enzymatic studies involving glycolysis and energy metabolism. Its recombinant form offers consistent activity and purity, making it suitable for high-throughput screening and industrial applications requiring reliable enzyme performance.