Lyticase recombinant is widely used in biotechnology and molecular biology for the enzymatic degradation of yeast cell walls. It is particularly valuable in the preparation of yeast spheroplasts, which are cells with partially removed cell walls, essential for various genetic manipulation techniques such as transformation and protoplast fusion. Additionally, it is employed in the extraction of intracellular components, including proteins and nucleic acids, due to its ability to efficiently break down the polysaccharide components of the yeast cell wall, such as beta-glucans and mannans. This enzyme is also utilized in studies involving yeast cell wall composition and function, aiding in the understanding of cell wall biosynthesis and its role in cell integrity. Its specificity and effectiveness make it a crucial tool in yeast-related research and industrial applications.