Acyl-CoA Synthetase plays a crucial role in lipid metabolism by activating fatty acids to form acyl-CoA, which is essential for energy production and biosynthesis. It is widely used in biochemical research to study fatty acid metabolism and lipid signaling pathways. In industrial applications, this enzyme is utilized in the production of biofuels, where it helps convert fatty acids into usable energy sources. Additionally, it is employed in the synthesis of various lipid-based compounds for pharmaceuticals and cosmetics. In metabolic engineering, Acyl-CoA Synthetase is used to enhance the production of fatty acid-derived products in microorganisms. Its function is also critical in understanding metabolic disorders related to fatty acid oxidation, aiding in the development of therapeutic strategies.