Aldolase is widely used in biochemical research and clinical diagnostics. It plays a crucial role in glycolysis, making it essential for studying metabolic pathways and energy production in cells. In clinical settings, aldolase levels are measured to diagnose and monitor muscle-related diseases, such as muscular dystrophy, as elevated levels can indicate muscle damage. Additionally, it is employed in enzymatic assays and biotechnology applications to catalyze specific reactions, aiding in the synthesis of complex molecules. Its role in metabolic studies also extends to understanding diseases like cancer, where altered glycolysis is a key feature.