5-Bromo-4-chloro-3-indolyl acetate
99%, esterase substrate
Reagent
Code: #99137
CAS Number
3252-36-6
blur_circular Chemical Specifications
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Molecular Information
Weight
288.53 g/mol
Formula
C₁₀H₇BrClNO₂
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Registry Numbers
MDL Number
MFCD00037932
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Physical Properties
Melting Point
106-107℃
Boiling Point
429℃
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Storage & Handling
Density
1.721
Storage
-20°C
description Product Description
Used primarily in biochemical and microbiological research as a substrate for enzyme assays, particularly for detecting esterase activity. When esterases act on it, the compound is hydrolyzed to produce an insoluble blue dye, which helps in visualizing enzyme activity in gel electrophoresis or histochemical staining. It is also employed in plant tissue culture studies to monitor cell viability and differentiation, as the enzymatic conversion provides a clear visual indicator of metabolic processes. Additionally, it serves as a reagent in molecular biology for detecting specific enzymatic reactions in various experimental setups.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Carbon | 41-42.2 |
| Nitrogen | 4.6-5.2 |
| Purity (HPLC) | 98.5-100 |
| Appearance | White to light beige powder |
| Solubility | Colorless to faint yellow, 50 mg/ml in ethanol |
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