5-Bromo-4-chloro-3-indolyl acetate

99%, esterase substrate

  • Product Code: 99137
  CAS:    3252-36-6
Molecular Weight: 288.53 g./mol Molecular Formula: C₁₀H₇BrClNO₂
EC Number: MDL Number: MFCD00037932
Melting Point: 106-107℃ Boiling Point: 429℃
Density: 1.721 Storage Condition: -20°C
Product Description: Used primarily in biochemical and microbiological research as a substrate for enzyme assays, particularly for detecting esterase activity. When esterases act on it, the compound is hydrolyzed to produce an insoluble blue dye, which helps in visualizing enzyme activity in gel electrophoresis or histochemical staining. It is also employed in plant tissue culture studies to monitor cell viability and differentiation, as the enzymatic conversion provides a clear visual indicator of metabolic processes. Additionally, it serves as a reagent in molecular biology for detecting specific enzymatic reactions in various experimental setups.
Product Specification:
Test Specification
Carbon 41-42.2
Nitrogen 4.6-5.2
Purity (HPLC) 98.5-100
Appearance White to light beige powder
Solubility Colorless to faint yellow, 50 mg/ml in ethanol
Sizes / Availability / Pricing:
Size Availability Price Quantity
25mg 10-20 days €51.39
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5-Bromo-4-chloro-3-indolyl acetate
Used primarily in biochemical and microbiological research as a substrate for enzyme assays, particularly for detecting esterase activity. When esterases act on it, the compound is hydrolyzed to produce an insoluble blue dye, which helps in visualizing enzyme activity in gel electrophoresis or histochemical staining. It is also employed in plant tissue culture studies to monitor cell viability and differentiation, as the enzymatic conversion provides a clear visual indicator of metabolic processes. Additionally, it serves as a reagent in molecular biology for detecting specific enzymatic reactions in various experimental setups.
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