Esterase from porcine liver is widely used in biochemical research and industrial applications due to its ability to catalyze the hydrolysis of ester bonds in various compounds. In the pharmaceutical industry, it is employed to synthesize and modify drug molecules, particularly in the production of chiral intermediates, which are crucial for developing enantiomerically pure drugs. In the food industry, this enzyme aids in flavor enhancement by breaking down esters to release aromatic compounds, improving the sensory qualities of products. Additionally, it is utilized in environmental biotechnology for the degradation of ester-based pollutants, contributing to waste management and bioremediation efforts. Its role in analytical chemistry includes serving as a tool for ester hydrolysis in sample preparation and assay development.