Esterase from porcine liver is widely utilized in biochemical research and industrial applications due to its ability to catalyze the hydrolysis of ester bonds. In the pharmaceutical industry, it is employed to study drug metabolism, particularly in the breakdown of ester-containing prodrugs into their active forms. This enzyme is also used in the food industry to enhance flavors and aromas by modifying ester compounds in various products, including breaking down esters that cause unpleasant odors and accelerating cheese ripening to develop flavor. Additionally, it plays a role in environmental biotechnology for degrading ester-based pollutants, contributing to bioremediation efforts. Its application in analytical chemistry includes its use as a reagent in assays to detect and quantify esterase activity in biological samples.