Esterase from porcine liver is widely utilized in biochemical research and industrial applications due to its ability to catalyze the hydrolysis of ester bonds. In the pharmaceutical industry, it is employed for the synthesis and modification of drug compounds, particularly in the production of chiral intermediates, where it helps achieve high enantioselectivity. In the food industry, this enzyme is used to enhance flavors by breaking down esters into their constituent alcohols and acids, contributing to the development of desirable aromas and tastes. Additionally, it plays a role in environmental biotechnology for the degradation of ester-containing pollutants, aiding in bioremediation processes. Its application in diagnostic assays is also notable, where it serves as a marker enzyme for detecting specific biochemical pathways or disease conditions.