10-Nitrooleate is primarily used in research settings to study the effects of nitro fatty acids on cellular signaling pathways. It serves as a model compound to investigate the role of nitro fatty acids in modulating inflammation, oxidative stress, and metabolic regulation. Researchers utilize it to explore its potential therapeutic applications in conditions such as cardiovascular diseases, diabetes, and inflammatory disorders. Additionally, it is employed in biochemical assays to understand its interactions with proteins and enzymes, particularly those involved in redox signaling. Its application extends to the development of novel anti-inflammatory and antioxidant agents, making it a valuable tool in biomedical research.