Eicosatetraynoic acid is primarily used in biochemical research to study the mechanisms of inflammation and cellular signaling pathways. It acts as an inhibitor of enzymes like lipoxygenase and cyclooxygenase, which are involved in the production of inflammatory mediators such as prostaglandins and leukotrienes. This makes it a valuable tool for investigating the role of these pathways in diseases like arthritis, asthma, and other inflammatory conditions. Additionally, it is utilized in studies exploring lipid metabolism and the impact of fatty acids on cellular functions. Its ability to modulate enzyme activity helps researchers understand the complex interactions within biological systems and develop potential therapeutic strategies.