Tryptone Powder Overview:
Tryptone powder is a peptone derived from the enzymatic digestion of casein, primarily used as a nutrient-rich component in microbiological culture media.
Key Features:
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Source: Derived from enzymatic hydrolysis of milk protein (casein).
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Composition: Rich in amino acids, peptides, and vitamins, particularly tryptophan.
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Appearance: Off-white to pale yellow, water-soluble powder.
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pH (1% solution): Typically around 6.5 - 7.5.
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Solubility: Easily soluble in water, clear to slightly hazy solutions.
Applications in Microbiology:
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Growth Media: Used widely for growing bacteria, fungi, and yeast.
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LB Broth: A key ingredient in Luria-Bertani broth for cultivating E. coli and other bacterial strains in genetic engineering and molecular biology.
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Fermentation: Provides essential nitrogen and growth factors for industrial fermentation processes.
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Diagnostic Media: Included in selective and differential media for microbial identification.
Preparation & Usage:
Typical usage concentrations:
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General culture media: 5-20 g/L
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LB broth preparation: approximately 10 g/L tryptone, 5 g/L yeast extract, and 5-10 g/L NaCl.
Sterilize prepared media by autoclaving at 121°C for 15-20 minutes.
Storage & Stability:
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Store at room temperature in a dry, sealed container.
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Protect from moisture and contamination.
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Shelf-life typically 2-3 years if stored properly.
Regulatory & Safety:
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Non-hazardous, food-grade standard, widely recognized as safe for microbiological use.
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Follow standard laboratory practices for handling powders and culture media.
